Difference between revisions of "Part:BBa K2598005:Design"
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Tabor, J. J., Levskaya, A. & Voigt, C. A. Multichromatic control of gene expression in Escherichia coli. J | Tabor, J. J., Levskaya, A. & Voigt, C. A. Multichromatic control of gene expression in Escherichia coli. J | ||
Mol Biol 405, 315-324, (2011). | Mol Biol 405, 315-324, (2011). | ||
| + | |||
| + | Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017) | ||
Latest revision as of 10:06, 13 October 2018
CcaS_CcaR Green light sensor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 676 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2774
Illegal XhoI site found at 2322 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1340
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence is optimized to delete illegal restriction sites and render it PFC compatible.
Source
It's from the genome of Synechocystis sp. PCC6803.
References
Tabor, J. J., Levskaya, A. & Voigt, C. A. Multichromatic control of gene expression in Escherichia coli. J Mol Biol 405, 315-324, (2011).
Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017)