Difference between revisions of "Part:BBa K2623021"
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<partinfo>BBa_K2623021 short</partinfo> | <partinfo>BBa_K2623021 short</partinfo> | ||
===SUMMARY=== | ===SUMMARY=== | ||
| − | This part | + | This part is a relatively basic part designed to target the archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). Porin outer-membrane protein A, OmpA, is a kind of highly expressed transmembrane porin protein of E. coli and is therefore abundant in OMVs. We inserted SpyTag to its N-termini to bioconjugate with the SpyCather in the following parts. to L7Ae (BBa_K2623006) in order to anchore L7Ae to outer membrane through the bioconjugation of the SpyTag/SpyCatcher system. Through the fluorescence of GFP, we can evaluate the efficiency of their transport to the outer membrane and OMVs. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 07:29, 13 October 2018
Bacterial outer membrane protein A (OmpA) fused with SpyTag at its N-termini and GFP at its C-termin
SUMMARY
This part is a relatively basic part designed to target the archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). Porin outer-membrane protein A, OmpA, is a kind of highly expressed transmembrane porin protein of E. coli and is therefore abundant in OMVs. We inserted SpyTag to its N-termini to bioconjugate with the SpyCather in the following parts. to L7Ae (BBa_K2623006) in order to anchore L7Ae to outer membrane through the bioconjugation of the SpyTag/SpyCatcher system. Through the fluorescence of GFP, we can evaluate the efficiency of their transport to the outer membrane and OMVs.
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 65
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1302