Difference between revisions of "Part:BBa K2686005:Experience"
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| − | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | + | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter how you used this part and how it worked out.The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing. |
| − | how you used this part and how it worked out.The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing. | + | |
===Applications of BBa_K2686005=== | ===Applications of BBa_K2686005=== | ||
Revision as of 18:00, 12 October 2018
The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing. The part was expressed in the pET vector using a cell-free expression system (See results on EPFL_2018 team wiki). The part was then amplified out of the pET vector and inserted into the pSB1C3 plasmid backbone, and submitted. There was not enough time to perform Sanger sequencing, but colony PCR gave us the correct insert size.
This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing.
Applications of BBa_K2686005
User Reviews
UNIQ16cb1f4f80ca5859-partinfo-00000000-QINU UNIQ16cb1f4f80ca5859-partinfo-00000001-QINU