Difference between revisions of "Part:BBa K2686006:Experience"
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The part was then amplified out of the pET vector and inserted into the pSB1C3 plasmid backbone, and submitted. There was not enough time to perform Sanger sequencing, but colony PCR gave us the correct insert size.
 
The part was then amplified out of the pET vector and inserted into the pSB1C3 plasmid backbone, and submitted. There was not enough time to perform Sanger sequencing, but colony PCR gave us the correct insert size.
  
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
  
 
===Applications of BBa_K2686006===
 
===Applications of BBa_K2686006===

Revision as of 17:55, 12 October 2018

The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing. The part was expressed in the pET vector using a BL21 DE3 cell free expression system. After expression and purification, the part was validated using a native gel, with multiple positive and negative controls (review iGEM EPFL 2018 results page).


The part was then amplified out of the pET vector and inserted into the pSB1C3 plasmid backbone, and submitted. There was not enough time to perform Sanger sequencing, but colony PCR gave us the correct insert size.


Applications of BBa_K2686006

User Reviews

UNIQce5c227ea5d7db47-partinfo-00000000-QINU UNIQce5c227ea5d7db47-partinfo-00000001-QINU