Difference between revisions of "Part:BBa K2686005:Experience"
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| − | The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing. The part was then amplified out of the pET vector and inserted into the pSB1C3 plasmid backbone, and submitted. There was not enough time to perform Sanger sequencing, but colony PCR gave us the correct insert size. | + | The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing. The part was expressed in the pET vector using a cell-free expression system (See results on EPFL_2018 team wiki). |
| + | The part was then amplified out of the pET vector and inserted into the pSB1C3 plasmid backbone, and submitted. There was not enough time to perform Sanger sequencing, but colony PCR gave us the correct insert size. | ||
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Revision as of 17:49, 12 October 2018
The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing. The part was expressed in the pET vector using a cell-free expression system (See results on EPFL_2018 team wiki). The part was then amplified out of the pET vector and inserted into the pSB1C3 plasmid backbone, and submitted. There was not enough time to perform Sanger sequencing, but colony PCR gave us the correct insert size.
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing.
Applications of BBa_K2686005
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UNIQe77e41efe78861e2-partinfo-00000000-QINU UNIQe77e41efe78861e2-partinfo-00000001-QINU