Difference between revisions of "Part:BBa K2607002:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 
To be added
 
To be added
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+
This part is assembled as improvement of B0034-Blue Florescent Protein (BFP) (<a href="https://parts.igem.org/Part:BBa_K592024">BBa_K592024</a>). Our established part consists of these following features:
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+
<ul>
 +
<li>lacI regulated promoter (BBa_R0010) in the upstream</li>
 +
<li>Double terminator (BBa_B0010 and BBa_B0012) in the downstream</li>
 +
<li>SalI restriction site between the promoter and ribosome binding site</li>
 +
<li>NdeI restriction site between the ribosome binding site and coding sequence</li>
 +
<li>Modified blue fluorescence protein coding sequence to eliminate SalI restriction site in the middle without altering amino acid sequence</li></ul>
  
 
===Source===
 
===Source===

Revision as of 16:27, 12 October 2018


Improved Blue Fluorescent Protein (BFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To be added This part is assembled as improvement of B0034-Blue Florescent Protein (BFP) (<a href="https://parts.igem.org/Part:BBa_K592024">BBa_K592024</a>). Our established part consists of these following features:

  • lacI regulated promoter (BBa_R0010) in the upstream
  • Double terminator (BBa_B0010 and BBa_B0012) in the downstream
  • SalI restriction site between the promoter and ribosome binding site
  • NdeI restriction site between the ribosome binding site and coding sequence
  • Modified blue fluorescence protein coding sequence to eliminate SalI restriction site in the middle without altering amino acid sequence

Source

To be added

References