Difference between revisions of "Part:BBa K2549007:Design"

 
m
 
(5 intermediate revisions by 2 users not shown)
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2549007 short</partinfo>
 
<partinfo>BBa_K2549007 short</partinfo>
−
 
 
<partinfo>BBa_K2549007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2549007 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
−
This part is the mouse Notch1 extended regulatory region.
+
This part is the mouse Notch1 extended regulatory region<ref>Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012</ref>. It was used as the transmembrane core domain of the SynNotch. However, original reported design has a high background activation in transfected 293T cells. We have put a lot of effect in optimizing this critical transmembrane domain for Notch receptor. Comparing with [[Part:BBa_K2549006]], minimal regulatory region (Ile1427 to Arg1752), extended regulatory region (Pro1390 to Arg1752) includes additional '''3''' EGF repeats.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
−
 
+
From IDT (gBlock), codon optimized for human
−
IDT(gBlock)
+
  
 
===References===
 
===References===

Latest revision as of 13:14, 12 October 2018


mouse Notch1 core (extended)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 703


Design Notes

This part is the mouse Notch1 extended regulatory region[1]. It was used as the transmembrane core domain of the SynNotch. However, original reported design has a high background activation in transfected 293T cells. We have put a lot of effect in optimizing this critical transmembrane domain for Notch receptor. Comparing with Part:BBa_K2549006, minimal regulatory region (Ile1427 to Arg1752), extended regulatory region (Pro1390 to Arg1752) includes additional 3 EGF repeats.

Source

From IDT (gBlock), codon optimized for human

References

  1. ↑ Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012