Difference between revisions of "Part:BBa K2549003:Design"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2549003 short</partinfo>
 
<partinfo>BBa_K2549003 short</partinfo>
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<partinfo>BBa_K2549003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2549003 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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Two copies of LaG16([[Part:Part:BBa_K2549002]]) were fused to form LaG2 using a flexible glycine-rich peptide linker<ref>A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60  PMID: 25362362; DOI: 10.1038/nmeth.3170</ref>.
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Two copies of LaG16 were fused to form LaG2 ([[Part:Part:BBa_K2549002]]) using a flexible glycine-rich peptide linker<ref>A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60  PMID: 25362362; DOI: 10.1038/nmeth.3170</ref>.
  
 
===Source===
 
===Source===
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IDT(gBlock)
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From IDT (gBlock), codon optimized for human
  
 
===References===
 
===References===

Latest revision as of 13:13, 12 October 2018


anti-GFP (LaG16)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 243
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Two copies of LaG16 were fused to form LaG2 (Part:Part:BBa_K2549002) using a flexible glycine-rich peptide linker[1].

Source

From IDT (gBlock), codon optimized for human

References

  1. ↑ A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170