Difference between revisions of "Part:BBa K2549001:Design"

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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2549001 short</partinfo>
 
<partinfo>BBa_K2549001 short</partinfo>
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<partinfo>BBa_K2549001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2549001 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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None.
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Surface-expressed CD19 (surCD19) is built by joining CD8&alpha; signal peptide, CD19 extracellular domain and the transmembrane region of PDGFR&beta; (from N terminal to C terminal). Additional HA tag on its N terminal and a Myc tag on its C terminal to facilitate detection by antibodies <ref>Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012</ref>. It was designed and used similarly as [[Part:BBa_K2446051]].
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===Source===
 
===Source===
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From IDT (gBlock), codon optimized for human
  
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from IDT(gBlock)
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=== Reference ===
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===References===
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[1]Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012
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Latest revision as of 13:11, 12 October 2018


suface-expressed CD19


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 588
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 757
    Illegal SapI site found at 273
    Illegal SapI.rc site found at 91


Design Notes

Surface-expressed CD19 (surCD19) is built by joining CD8α signal peptide, CD19 extracellular domain and the transmembrane region of PDGFRβ (from N terminal to C terminal). Additional HA tag on its N terminal and a Myc tag on its C terminal to facilitate detection by antibodies [1]. It was designed and used similarly as Part:BBa_K2446051.

Source

From IDT (gBlock), codon optimized for human

Reference

  1. ↑ Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012