Difference between revisions of "Part:BBa K2547000:Experience"
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&nbsp;&nbsp;&nbsp;&nbsp;We first synthesized the sequence of human carbonic anhydrase CA2, and then cloned it into the expression vector pET-30a(+), and identified the correctness of the obtained recombinant vector by restriction enzyme digestion and sequencing (Fig. 1 and Fig. 2).<br></p>
 
&nbsp;&nbsp;&nbsp;&nbsp;We first synthesized the sequence of human carbonic anhydrase CA2, and then cloned it into the expression vector pET-30a(+), and identified the correctness of the obtained recombinant vector by restriction enzyme digestion and sequencing (Fig. 1 and Fig. 2).<br></p>
 
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<p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 14px;text-align: center;">Fig. 6 SDS-PAGE and Western blot analysis of CA2. Lane 1: Positive control (BSA); Lane 2: purified CA2; Lane 3: Purified CA2.
 
<p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆';  font-size: 14px;text-align: center;">Fig. 6 SDS-PAGE and Western blot analysis of CA2. Lane 1: Positive control (BSA); Lane 2: purified CA2; Lane 3: Purified CA2.
 
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Revision as of 12:13, 12 October 2018


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UNIQ6ce4dc7a712085db-partinfo-00000000-QINU UNIQ6ce4dc7a712085db-partinfo-00000001-QINU