Difference between revisions of "Part:BBa K2856002"
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| − | ===Usage and Biology=== | + | ===Usage and Biology=== |
| + | S-adenosylmethionine synthetase(MetK) is an enzyme involved and responded to synthetic reaction of GSH.In this | ||
| + | reaction,one Cys and one Glu are converted to one γ-GC,then one γ-GC and one Gly are converted to one GSH.(Figure 1) The Lactococcus Lactis NZ9000 has inability to synthesis GSH.In our project,we construct a plasmid harboring gshF in order to produnce GSH in Lactococcus Lactis NZ9000. | ||
| + | |||
[[File:T--H14Z1 Hangzhou--Reaction_metK.jpeg|500px|thumb|centre| <p>'''Figure. 1 Enzymatic reaction catalyzed by metK'''</p>]] | [[File:T--H14Z1 Hangzhou--Reaction_metK.jpeg|500px|thumb|centre| <p>'''Figure. 1 Enzymatic reaction catalyzed by metK'''</p>]] | ||
Revision as of 08:44, 12 October 2018
Modification of S-adenosylmethionine synthetase (MetK)
The BBa_K2856001 harbors a coding sequence of S-adenosylmethionine synthetase(MetK) derived from Lactococcus Lactis NZ9000 genome.The MetK protein catalyzes methionine and ATP to S-adenosyl methionine.
Usage and Biology
S-adenosylmethionine synthetase(MetK) is an enzyme involved and responded to synthetic reaction of GSH.In this reaction,one Cys and one Glu are converted to one γ-GC,then one γ-GC and one Gly are converted to one GSH.(Figure 1) The Lactococcus Lactis NZ9000 has inability to synthesis GSH.In our project,we construct a plasmid harboring gshF in order to produnce GSH in Lactococcus Lactis NZ9000.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]