Difference between revisions of "Part:BBa K2598052"
Line 20: Line 20:
 
===Characterization===
 
===Characterization===
 
<b>Figure 1</b> shows the relationship between the wavelength of light exposed on liquid medium and the intensity of BFP, GFP and RFP E. coli expressed from left figure to right figure respectively. We got the data through flow cytometer and analyzed it to get the figure. The y-axis is the number of cells, and the x-axis is fluorescence intensity. And every color is E. coli that grows for 8 hours under the light of the corresponding wavelength. We can see E. coli has the highest blue fluorescence expression under blue light from the left graph. And We can also see E. coli has the highest green and red fluorescence expression under green light and right light from the middle and right graph respectively. So this figure proves that our system and our parts can work well.
 
<b>Figure 1</b> shows the relationship between the wavelength of light exposed on liquid medium and the intensity of BFP, GFP and RFP E. coli expressed from left figure to right figure respectively. We got the data through flow cytometer and analyzed it to get the figure. The y-axis is the number of cells, and the x-axis is fluorescence intensity. And every color is E. coli that grows for 8 hours under the light of the corresponding wavelength. We can see E. coli has the highest blue fluorescence expression under blue light from the left graph. And We can also see E. coli has the highest green and red fluorescence expression under green light and right light from the middle and right graph respectively. So this figure proves that our system and our parts can work well.
−
<div>[[File:T—UCAS-China—LIGHT AND LIGHT.png |1000px|thumb|center|<b>Figure 1:</b>Relationship between the wavelength of light exposed on liquid medium and the intensity of BFP, GFP and RFP E. coli expressed from left figure to right figure respectively]]</div>
+
<div>[[File:T—UCAS-China—LIGHT AND LIGHT.png |700px|thumb|center|<b>Figure 1:</b>Relationship between the wavelength of light exposed on liquid medium and the intensity of BFP, GFP and RFP E. coli expressed from left figure to right figure respectively]]</div>
  
 
===Characterization===
 
===Characterization===
 
<b>Figure 2</b> shows the relationship between fluorescence intensity and excitation wavelength. The x-axis is wavelength of 10h illumination. The solid medium gradually emerged and the y-axis is RGB figure of fluorescence in illuminated solid medium. This curve illustrates how our system responses to different excitation wavelength, which perfectly meets our expectation. So this figure proves that our system and our parts can work well.
 
<b>Figure 2</b> shows the relationship between fluorescence intensity and excitation wavelength. The x-axis is wavelength of 10h illumination. The solid medium gradually emerged and the y-axis is RGB figure of fluorescence in illuminated solid medium. This curve illustrates how our system responses to different excitation wavelength, which perfectly meets our expectation. So this figure proves that our system and our parts can work well.
−
<div>[[File:T—UCAS-China—abc222.png|1000px|thumb|center|<b>Figure 2:</b>Relationship between fluorescence intensity and excitation wavelength]]</div>
+
<div>[[File:T—UCAS-China—abc222.png|700px|thumb|center|<b>Figure 2:</b>Relationship between fluorescence intensity and excitation wavelength]]</div>
  
 
===Characterization===
 
===Characterization===
 
<b>Figure 3</b> shows colors we got from the solid medium exposed under light, in which E. coli producing fluorescent protein grows.  When E .coli producing fluorescent protein are exposed under uniform light of single wavelength, the solid medium gradually emerged corresponding colors. And using color picker, we get many pure colors with predominant continuity.
 
<b>Figure 3</b> shows colors we got from the solid medium exposed under light, in which E. coli producing fluorescent protein grows.  When E .coli producing fluorescent protein are exposed under uniform light of single wavelength, the solid medium gradually emerged corresponding colors. And using color picker, we get many pure colors with predominant continuity.
−
<div>[[File:T—UCAS-China—FP SOLIDE.png|1000px|thumb|center|<b>Figure 3:</b> Colors we got from the solid medium, in which E. coli producing fluorescent protein grows, exposed under light]]</div>
+
<div>[[File:T—UCAS-China—FP SOLIDE.png|700px|thumb|center|<b>Figure 3:</b> Colors we got from the solid medium, in which E. coli producing fluorescent protein grows, exposed under light]]</div>
 +
 
 +
===Characterization===
 +
We explored the relationship between fluorescent intensity and illumination intensity, which affects the shade of the color.
 +
<b>Figure 4</b> shows the red fluorescent intensity of ecoli under  light of 620-630nm wavelength with different illumination intensity. We found when illuminated under around 400-600lux light, we can get the most red fluorescence. We also explored the relationship between green and blue  fluorescent intensity of ecoli under  light of 515-530nm wavelength and 460-470nm wavelength respectively and illumination intensity. The results were similar, that is, moderate intensity of light is most favorable for E. coli to express fluorescence.
 +
<div>[[File:T—UCAS-China—RR2018.png|700px|thumb|center|<b>Figure 4:</b>The relationship between the red fluorescent intensity of ecoli under light of 620-630nm wavelength and illumination intensity]]</div>

Revision as of 06:41, 12 October 2018


lacZ+bFMO+gusA

This part contains lacZ, bFMO and gusA under the regulation of T7 RNAPsigma fragment.We use this part as output signal and our 'paint'

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3112
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 6208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5
    Illegal AgeI site found at 3879
    Illegal AgeI site found at 4209
    Illegal AgeI site found at 4513
    Illegal AgeI site found at 4536
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Figure 1 shows the relationship between the wavelength of light exposed on liquid medium and the intensity of BFP, GFP and RFP E. coli expressed from left figure to right figure respectively. We got the data through flow cytometer and analyzed it to get the figure. The y-axis is the number of cells, and the x-axis is fluorescence intensity. And every color is E. coli that grows for 8 hours under the light of the corresponding wavelength. We can see E. coli has the highest blue fluorescence expression under blue light from the left graph. And We can also see E. coli has the highest green and red fluorescence expression under green light and right light from the middle and right graph respectively. So this figure proves that our system and our parts can work well.

Figure 1:Relationship between the wavelength of light exposed on liquid medium and the intensity of BFP, GFP and RFP E. coli expressed from left figure to right figure respectively

Characterization

Figure 2 shows the relationship between fluorescence intensity and excitation wavelength. The x-axis is wavelength of 10h illumination. The solid medium gradually emerged and the y-axis is RGB figure of fluorescence in illuminated solid medium. This curve illustrates how our system responses to different excitation wavelength, which perfectly meets our expectation. So this figure proves that our system and our parts can work well.

Figure 2:Relationship between fluorescence intensity and excitation wavelength

Characterization

Figure 3 shows colors we got from the solid medium exposed under light, in which E. coli producing fluorescent protein grows. When E .coli producing fluorescent protein are exposed under uniform light of single wavelength, the solid medium gradually emerged corresponding colors. And using color picker, we get many pure colors with predominant continuity.

Figure 3: Colors we got from the solid medium, in which E. coli producing fluorescent protein grows, exposed under light

Characterization

We explored the relationship between fluorescent intensity and illumination intensity, which affects the shade of the color. Figure 4 shows the red fluorescent intensity of ecoli under light of 620-630nm wavelength with different illumination intensity. We found when illuminated under around 400-600lux light, we can get the most red fluorescence. We also explored the relationship between green and blue fluorescent intensity of ecoli under light of 515-530nm wavelength and 460-470nm wavelength respectively and illumination intensity. The results were similar, that is, moderate intensity of light is most favorable for E. coli to express fluorescence.

Figure 4:The relationship between the red fluorescent intensity of ecoli under light of 620-630nm wavelength and illumination intensity