Difference between revisions of "Part:BBa K2789009:Design"

Revision as of 05:50, 12 October 2018

optimized Ccas from BBa_K68102

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1310
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 715
Illegal AgeI site found at 1720
Illegal AgeI site found at 1915
1000
COMPATIBLE WITH RFC[1000]

Design Notes

We have optimized the code in the Ccas and we deleted the EcoRI restriction site in the Ccas for future DNA ligation and digestion.

Source

initial Ccas from BBa_K68102

References

We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence. We paste the website of BBa_K608102 (https://parts.igem.org/Part:BBa_K608102:Design)

Revision as of 05:44, 12 October 2018 (view source) Duliaoqi (Talk \| contribs) (→‎References) ← Older edit		Revision as of 05:50, 12 October 2018 (view source) Duliaoqi (Talk \| contribs) (→‎References) Newer edit →
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	===References===		===References===
−	We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence. We paste the website of BBa_K608102 (https://parts.igem.org/Part:BBa_K608102)	+	We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence. We paste the website of BBa_K608102 (https://parts.igem.org/Part:BBa_K608102:Design)