Difference between revisions of "Part:BBa K2683007"

Latest revision as of 03:30, 12 October 2018

DecS134C codon optimized for E.coli

DecS134C is a mutated form of a protein originally from the phage L genome. It forms a trimer dimer when presented with protein nanocompartments as seen in previous literature [1]. The mutation of serine to cysteine causes the interactions between the other DecS134C which causes connections of dimers that can form higher order structures[1]. This part is optimized for expression in E.coli and has a hexahistidine tag on the N-terminus for purification.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 36
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2683007 short</partinfo>
-DecS134C is a mutated form of a protein originally from the phage L genome. It forms a trimer dimer when presented with protein nanocompartments as seen in previous literature [1]. The mutation of serine to cysteine causes the interactions between the other DecS134C which causes connections of dimers that can form higher order structures[1]. This part is optimized for expression in <i> E.coli </i> and has a his tag on the N-terminus.
+DecS134C is a mutated form of a protein originally from the phage L genome. It forms a trimer dimer when presented with protein nanocompartments as seen in previous literature [1]. The mutation of serine to cysteine causes the interactions between the other DecS134C which causes connections of dimers that can form higher order structures[1]. This part is optimized for expression in <i> E.coli </i> and has a hexahistidine tag on the N-terminus for purification.
 <!-- Add more about the biology of this part here