Difference between revisions of "Part:BBa K2683007"
Kristiturton (Talk | contribs) |
|||
(3 intermediate revisions by the same user not shown) | |||
Line 3: | Line 3: | ||
<partinfo>BBa_K2683007 short</partinfo> | <partinfo>BBa_K2683007 short</partinfo> | ||
− | DecS134C is a mutated form of a protein originally from the phage L genome. It forms a trimer dimer when presented with protein nanocompartments as seen in previous literature [ | + | DecS134C is a mutated form of a protein originally from the phage L genome. It forms a trimer dimer when presented with protein nanocompartments as seen in previous literature [1]. The mutation of serine to cysteine causes the interactions between the other DecS134C which causes connections of dimers that can form higher order structures[1]. This part is optimized for expression in <i> E.coli </i> and has a hexahistidine tag on the N-terminus for purification. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 03:30, 12 October 2018
DecS134C codon optimized for E.coli
DecS134C is a mutated form of a protein originally from the phage L genome. It forms a trimer dimer when presented with protein nanocompartments as seen in previous literature [1]. The mutation of serine to cysteine causes the interactions between the other DecS134C which causes connections of dimers that can form higher order structures[1]. This part is optimized for expression in E.coli and has a hexahistidine tag on the N-terminus for purification.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 36
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]