Difference between revisions of "Part:BBa K2683007"
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<partinfo>BBa_K2683007 short</partinfo> | <partinfo>BBa_K2683007 short</partinfo> | ||
− | DecS134C is a mutated form of a protein originally from the phage L genome. It forms a trimer dimer when presented with protein nanocompartments as seen in previous literature [1]. The mutation of serine to cysteine causes the interactions between the other | + | DecS134C is a mutated form of a protein originally from the phage L genome. It forms a trimer dimer when presented with protein nanocompartments as seen in previous literature [1]. The mutation of serine to cysteine causes the interactions between the other DecS134C which causes connections of dimers that can form higher order structures[1]. This part is optimized for expression in <i> E.coli </i> and has a his tag on the N-terminus. |
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Revision as of 03:30, 12 October 2018
DecS134C codon optimized for E.coli
DecS134C is a mutated form of a protein originally from the phage L genome. It forms a trimer dimer when presented with protein nanocompartments as seen in previous literature [1]. The mutation of serine to cysteine causes the interactions between the other DecS134C which causes connections of dimers that can form higher order structures[1]. This part is optimized for expression in E.coli and has a his tag on the N-terminus.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 36
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]