Difference between revisions of "Part:BBa K2683000:Design"

 
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===Design Notes===
 
===Design Notes===
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All designs of this construct were based on its homology with HIV. There were no additional sequences or tags used or considered
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All designs of this construct were based on its homology with HIV. This part was codon optimized for use in <i E.coli </i>
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===Source===
 
===Source===
  
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This part is a theoretical construct. The sequence is derived from the Arc protein found in mouse and was chosen based on its homology with HIV  
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This part is a theoretical construct. The sequence is derived from the Arc protein found in rat and was chosen based on its homology with HIV. https://www.uniprot.org/uniprot/Q63053
  
 
===References===
 
===References===
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[1]Pastuzyn, E., Day, C., Keams, R., Kyrke-Smith, M., Taibi, A., McCormick, J., Yoder, N., Belnap, D., Erlendsson, S., Morado, D., Briggs, J., Feschotte, C., and Shepard, J. (2018) The Neuronal Gene <i> Arc </i> encodes a repurposed retrotransposon Gag protein that mediates intercellular RNA transfer. cell. 172, 275-288

Latest revision as of 03:02, 12 October 2018


Arc minimal protein construct derived from HIV homology


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All designs of this construct were based on its homology with HIV. This part was codon optimized for use in <i E.coli </i>

Source

This part is a theoretical construct. The sequence is derived from the Arc protein found in rat and was chosen based on its homology with HIV. https://www.uniprot.org/uniprot/Q63053

References

[1]Pastuzyn, E., Day, C., Keams, R., Kyrke-Smith, M., Taibi, A., McCormick, J., Yoder, N., Belnap, D., Erlendsson, S., Morado, D., Briggs, J., Feschotte, C., and Shepard, J. (2018) The Neuronal Gene Arc encodes a repurposed retrotransposon Gag protein that mediates intercellular RNA transfer. cell. 172, 275-288