Difference between revisions of "Part:BBa K2683028"

Latest revision as of 01:56, 12 October 2018

MyoKinase (MK) in pSB1C3

Myokinase (MK) is a enzyme that transfers phosphate goups in order to convert adenine nucleotides (ATP, ADP, and AMP). Due to its function, this protein is essential for maintaining cellular homeostasis and energy levels [1]. This part is codon optimized for use in E.coli , has a N-terminal His tag and has a T7 promoter, medium strength RBS and double terminator. This part was originally designed by the 2017 Lethbridge iGEM team ( http://2017.igem.org/Team:Lethbridge). The part was initially characterized in the pUC57 plasmid (https://parts.igem.org/Part:BBa_K2443033).

This part was improved by placing it into the pSB1C3 plasmid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 749
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 Myokinase (MK) is a enzyme that transfers phosphate goups in order to convert adenine nucleotides (ATP, ADP, and AMP). Due to its function, this protein is essential for maintaining cellular homeostasis and energy levels [1]. This part is codon optimized for use in <i> E.coli </i>, has a N-terminal His tag and has a T7 promoter, medium strength RBS and double terminator. This part was originally designed by the 2017 Lethbridge iGEM team ( http://2017.igem.org/Team:Lethbridge). The part was initially characterized in the pUC57 plasmid (https://parts.igem.org/Part:BBa_K2443033).
-This part was improved by placing it into the pSbB1C3 plasmid.
+This part was improved by placing it into the pSB1C3 plasmid.
 <!-- Add more about the biology of this part here