Difference between revisions of "Part:BBa K2694001:Design"
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Wilhelm et al. (1999). Synthesized through BioBasic. | Wilhelm et al. (1999). Synthesized through BioBasic. | ||
| − | ===References== | + | ===References=== |
Wilhelm S., Thomassen J., & Jaeger K.E. (1999). A Novel Lipolytic Enzyme Located in the Outer Membrane of Pseudomonas aeruginosa. Journal of Bacteriology. 6977-86. | Wilhelm S., Thomassen J., & Jaeger K.E. (1999). A Novel Lipolytic Enzyme Located in the Outer Membrane of Pseudomonas aeruginosa. Journal of Bacteriology. 6977-86. | ||
Latest revision as of 00:47, 12 October 2018
EstA from Pseudomonas aeruginosa
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 215
Illegal BglII site found at 362 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 145
Illegal NgoMIV site found at 601
Illegal NgoMIV site found at 739
Illegal NgoMIV site found at 1279
Illegal NgoMIV site found at 1648 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was designed according to a paper by Wilhelm et al. in the Journal of Bacteriology in 1999 and the corresponding EBI sequence. This paper included the DNA sequence that coded the esterase, which we codon optimized and removed the illegal cut sites via silent mutations (i.e., the amino acid sequence was unchanged).
Source
Wilhelm et al. (1999). Synthesized through BioBasic.
References
Wilhelm S., Thomassen J., & Jaeger K.E. (1999). A Novel Lipolytic Enzyme Located in the Outer Membrane of Pseudomonas aeruginosa. Journal of Bacteriology. 6977-86.