Difference between revisions of "Part:BBa K2788010:Design"
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===References=== | ===References=== | ||
| + | [1]Xiao G, Ying S H, Peng Z, et al. Genomic perspectives on the evolution of fungal entomopathogenicity in Beauveria bassiana[J]. Scientific Reports, 2012, 2(483):483. | ||
| + | <div> | ||
| + | [2]Ye Zhang, Zhongren Lei, Haihong Wang, Jiqing Zhan. Prokaryotic expression and immunolocalization Beauveria bassiana HsbA protein [J] Chinese Agricultural Sciences, 2013,46 (21): 4534-4541. | ||
Latest revision as of 21:35, 11 October 2018
PgpdA-HsbA-TtrpC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 903
Illegal NheI site found at 2993 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 137
Illegal BamHI site found at 3059
Illegal XhoI site found at 693
Illegal XhoI site found at 1075 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2553
Illegal NgoMIV site found at 3695
Illegal AgeI site found at 1282
Illegal AgeI site found at 1430 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1109
Illegal BsaI.rc site found at 3409
Illegal SapI site found at 315
Design Notes
In order to facilitate the replacement of the gene of interest, we added HindIII and KpnI restriction sites on both sides of the gene of interest.
Source
PgpdA comes from BBa_K2040101 HsbA comes from BBa_K2788000 TtrpC comes from BBa_K2040102
References
[1]Xiao G, Ying S H, Peng Z, et al. Genomic perspectives on the evolution of fungal entomopathogenicity in Beauveria bassiana[J]. Scientific Reports, 2012, 2(483):483.
[2]Ye Zhang, Zhongren Lei, Haihong Wang, Jiqing Zhan. Prokaryotic expression and immunolocalization Beauveria bassiana HsbA protein [J] Chinese Agricultural Sciences, 2013,46 (21): 4534-4541.