Difference between revisions of "Part:BBa K2788011:Design"
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===References=== | ===References=== | ||
| + | [1]Kan Zhuo, Li Yu, Jinling Liao. Paecilomyces lilacinus MD1 chitinase activity assay [J] Agricultural Sciences, 2009 (05): 167-170. | ||
| + | <div> | ||
| + | [2]Xiaozhen Shi, Min Wang, Huaping Huang, Lijia Guo, Wei Song, Junsheng Huang. Analysis of Chitinase Activity of Metarhizium anisopliae and Its Correlation with Toxicity of Coleoptera[J]. Guangxi Agricultural Sciences,2008(03):313-316. | ||
Latest revision as of 21:34, 11 October 2018
PgpdA-Bbchit-TtrpC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 903
Illegal NotI site found at 2890 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 137
Illegal BamHI site found at 3365
Illegal XhoI site found at 693
Illegal XhoI site found at 1075
Illegal XhoI site found at 2877 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2559
Illegal NgoMIV site found at 3088
Illegal NgoMIV site found at 4001
Illegal AgeI site found at 1282
Illegal AgeI site found at 1430 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1109
Illegal BsaI.rc site found at 3715
Illegal SapI site found at 315
Design Notes
In order to facilitate the replacement of the gene of interest, we added HindIII and KpnI restriction sites on both sides of the gene of interest.
Source
PgpdA comes from BBa_K2040101 Bbchit comes from BBa_K2788001 TtrpC comes from BBa_K2040102
References
[1]Kan Zhuo, Li Yu, Jinling Liao. Paecilomyces lilacinus MD1 chitinase activity assay [J] Agricultural Sciences, 2009 (05): 167-170.
[2]Xiaozhen Shi, Min Wang, Huaping Huang, Lijia Guo, Wei Song, Junsheng Huang. Analysis of Chitinase Activity of Metarhizium anisopliae and Its Correlation with Toxicity of Coleoptera[J]. Guangxi Agricultural Sciences,2008(03):313-316.