Difference between revisions of "Part:BBa M31234:Design"

 
 
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<partinfo>BBa_M31234 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
−
Designing an appropriate linker  
+
Removed stop codon from Calcium channel sequence
 +
 
 +
Used the 13 amino acid linker sequence: ATT CGG TCC GGC GGG AGC GGA TCC GGC GGC CAG TCC CCG
 +
 
 +
Coding for Aequorea victoria begins with AATG
  
  

Latest revision as of 01:50, 14 May 2008

Calcium channel-apoaequorin fusion


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1717
    Illegal PstI site found at 431
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1717
    Illegal PstI site found at 431
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1717
    Illegal BamHI site found at 964
    Illegal BamHI site found at 1614
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1717
    Illegal PstI site found at 431
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1717
    Illegal PstI site found at 431
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 485
    Illegal BsaI.rc site found at 734


Design Notes

Removed stop codon from Calcium channel sequence

Used the 13 amino acid linker sequence: ATT CGG TCC GGC GGG AGC GGA TCC GGC GGC CAG TCC CCG

Coding for Aequorea victoria begins with AATG


Source

Accession number for Homo sapiens neuronal voltage gated calcium channel gamma-3 subunit = AF100346

Accession number for Aequorea victoria apoaequorin = E14214

References