Difference between revisions of "Part:BBa M31234:Design"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_M31234 short</partinfo>
 
<partinfo>BBa_M31234 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Designing an appropriate linker  
+
Removed stop codon from Calcium channel sequence
 +
 
 +
Used 39 amino acid linker sequence: ATT CGG TCC GGC GGG AGC GGA TCC GGC GGC CAG TCC CCG C
 +
 
 +
Coding for Aequorea victoria begins with AATG
  
  

Revision as of 01:42, 14 May 2008

Calcium channel-apoaequorin fusion


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1717
    Illegal PstI site found at 431
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1717
    Illegal PstI site found at 431
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1717
    Illegal BamHI site found at 964
    Illegal BamHI site found at 1614
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1717
    Illegal PstI site found at 431
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1717
    Illegal PstI site found at 431
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 485
    Illegal BsaI.rc site found at 734


Design Notes

Removed stop codon from Calcium channel sequence

Used 39 amino acid linker sequence: ATT CGG TCC GGC GGG AGC GGA TCC GGC GGC CAG TCC CCG C

Coding for Aequorea victoria begins with AATG


Source

Accession number for Homo sapiens neuronal voltage gated calcium channel gamma-3 subunit = AF100346

Accession number for Aequorea victoria apoaequorin = E14214

References