Difference between revisions of "Part:BBa K2591020"

Revision as of 17:41, 11 October 2018

A gRNA designed to PORCN, an essential gene to secretion of the canonical wnt protein

Usage and Biology

Single-gRNA is a small RNA which help CRISRR-Cas protein family to target the exogenous sequence, and assist the prokaryotes to defend phages. Nowadays, the artificial gRNA can achieve the modification casually in the gene level and is a excellent way to introduce mutation. In our project, we design this gRNA for porcupine O-acyltransferase (PORCN), an essential enzyme to function at the Wnt3A protein secretion. Therefore, we use the gRNA to target PORCN, we can inhibit the donor cell to secret the Wnt3A protein normally.

Characterization

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K2591020 short</partinfo>
+===Usage and Biology===
 Single-gRNA is a small RNA which help CRISRR-Cas protein family to target the exogenous sequence, and assist the prokaryotes to defend phages. Nowadays, the artificial gRNA can achieve the modification casually in the gene level and is a excellent way to introduce mutation. In our project, we design this gRNA for porcupine O-acyltransferase (PORCN), an essential enzyme to function at the Wnt3A protein secretion. Therefore, we use the gRNA to target PORCN, we can inhibit the donor cell to secret the Wnt3A protein normally.
-<!-- Add more about the biology of this part here
-===Usage and Biology===