Difference between revisions of "Part:BBa K2683016"

Revision as of 05:03, 11 October 2018

Release Factor 1 in pSB1C3

Release factor 1 (RF1) is a protein that terminates translation through the recognition of the stop codon in an mRNA sequence. RF1 was originally designed by Lethbridge iGEM team 2017 (http://2017.igem.org/Team:Lethbridge). The original page can be found here: https://parts.igem.org/Part:BBa_K2443029 . RF1 had been Codon optimized for use in Escherichia coli and contains a N-Terminal hexahistidine tag and is under the regulation of T7 Promoter, RBS and double terminator.

This year we have been able to place this composite part in the pSB1C3 standard plasmid and have sent it to the registry.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 675
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2683016 short</partinfo>
-release factor 1 that is improved by insertion into the pSB1C3 standard
+Release factor 1 (RF1) is a protein that terminates translation through the recognition of the stop codon in an mRNA sequence. RF1 was originally designed by Lethbridge iGEM team 2017 (http://2017.igem.org/Team:Lethbridge). The original page can be found here: https://parts.igem.org/Part:BBa_K2443029 . RF1 had been Codon optimized for use in Escherichia coli and contains a N-Terminal hexahistidine tag and is under the regulation of T7 Promoter, RBS and double terminator.
+This year we have been able to place this composite part in the pSB1C3 standard plasmid and have sent it to the registry.
 <!-- Add more about the biology of this part here