Difference between revisions of "Part:BBa K2665014"
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SseNHX1 | SseNHX1 | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
SseNHX1 is the vacuolar Na(+)/H(+) antiporter. This antiporter enables to survive high osmotic pressure by sequestering excessive cytoplasmic Na(+) into vacuole. | SseNHX1 is the vacuolar Na(+)/H(+) antiporter. This antiporter enables to survive high osmotic pressure by sequestering excessive cytoplasmic Na(+) into vacuole. | ||
Revision as of 03:29, 11 October 2018
TDH3 promoter->SseNHX1->6xHis->CYC terminator
SseNHX1
Usage and Biology
SseNHX1 is the vacuolar Na(+)/H(+) antiporter. This antiporter enables to survive high osmotic pressure by sequestering excessive cytoplasmic Na(+) into vacuole.
The gene, SseNHX1 is created by DNA family shuffling; Two homologous Na(+)/H(+) antiporters from halophytes Salicornia europaea (SeNHX1) and Suaeda salsa (SsNHX1) were shuffled.
By reference, SseNHX1 enables higher salt tolerance and sequester Na(+) than the two protein, SeNHX1 and SsNHX1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2379
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]