Difference between revisions of "Part:BBa K2692000:Design"
Kristiturton (Talk | contribs) (→Design Notes) |
Kristiturton (Talk | contribs) (→Design Notes) |
||
Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
− | The hexahistidine tag has the ability to also bind to metals (this is why is is able to bind to a nickle column). To gain accurate results, the his tag would have to be removed to ensure that the cutA protein is binding the copper. The coding sequence is codon optimized for efficient expression in <i> E.coli < | + | The hexahistidine tag has the ability to also bind to metals (this is why is is able to bind to a nickle column). To gain accurate results, the his tag would have to be removed to ensure that the cutA protein is binding the copper. The coding sequence is codon optimized for efficient expression in <i> E.coli </i> |
===Source=== | ===Source=== |
Revision as of 03:13, 11 October 2018
CutA metal binding protein from E.coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The hexahistidine tag has the ability to also bind to metals (this is why is is able to bind to a nickle column). To gain accurate results, the his tag would have to be removed to ensure that the cutA protein is binding the copper. The coding sequence is codon optimized for efficient expression in E.coli
Source
The part in plasmid was a gift from the Weiden lab at the University of Lethbridge and was a part of the ASKA database.