Difference between revisions of "Part:BBa K2692001"
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<partinfo>BBa_K2692001 short</partinfo> | <partinfo>BBa_K2692001 short</partinfo> | ||
− | gp23 is one of the two major capsid proteins that make up the head of the T4 bacteriophage. In order for the protein to fold into its proper structure, gp23 needs the help of a chaperone protein gp31, which was also designed by the team this year (https://parts.igem.org/Part:BBa_K2692003). The gp23 protein makes up a majority of the capsid, whereby it self assembles into 160 hexamers. This part has been codon optimized for use in <i> Escherichia coli <i/>. | + | gp23 is one of the two major capsid proteins that make up the head of the T4 bacteriophage. In order for the protein to fold into its proper structure, gp23 needs the help of a chaperone protein gp31 [1], which was also designed by the team this year (https://parts.igem.org/Part:BBa_K2692003). The gp23 protein makes up a majority of the capsid, whereby it self assembles into 160 hexamers. This part has been codon optimized for use in <i> Escherichia coli <i/>. |
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Revision as of 02:18, 11 October 2018
Major T4 Capsid Protein gp23
gp23 is one of the two major capsid proteins that make up the head of the T4 bacteriophage. In order for the protein to fold into its proper structure, gp23 needs the help of a chaperone protein gp31 [1], which was also designed by the team this year (https://parts.igem.org/Part:BBa_K2692003). The gp23 protein makes up a majority of the capsid, whereby it self assembles into 160 hexamers. This part has been codon optimized for use in Escherichia coli <i/>.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 9
Illegal PstI site found at 342
Illegal PstI site found at 1524 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 9
Illegal PstI site found at 342
Illegal PstI site found at 1524
Illegal NotI site found at 15 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 9
Illegal BamHI site found at 1069 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 9
Illegal PstI site found at 342
Illegal PstI site found at 1524 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 9
Illegal XbaI site found at 24
Illegal PstI site found at 342
Illegal PstI site found at 1524 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1118