Difference between revisions of "Part:BBa K2739009"
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<partinfo>BBa_K2739009 short</partinfo>
 
<partinfo>BBa_K2739009 short</partinfo>
  
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This composite part is designed to investigate the effect of PHBV production using PHA synthetic pathway (PHA operon) with BtkB co-expressed. The BktB was isolated from R. eutropha H16 and being recognised as a phaA paralogous, which allows the formation of 3-ketovaleryl-CoA and leads to PHBV production.  
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This is a composite part that was created to allow us to test the functionality of BktB. PHA operon is known to produce bioplastic PHB. The coexpression of the BktB allow the production of copolymer, PHBV.  
  
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===Usage and Biology===
  
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The BktB was isolated from R. eutropha H16 and being recognised as a phaA paralogous, which allows the formation of 3-ketovaleryl-CoA and leads to PHBV production. In order to enhance the 3HV fraction in PHBV, paralog bktB was introduced into E. coli BL21 (DE3) with co-expression of phaCAB operon from Ralstonia eutropha.  
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===Usage and Biology===
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In order to enhance the 3HV fraction in PHBV, paralog bktB was introduced into E. coli BL21 (DE3) with co-expression of phaCAB operon from Ralstonia eutropha.  
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https://static.igem.org/mediawiki/parts/8/85/T--Edinburgh_OG_BBa_K2739009--image_1.jpg
 
https://static.igem.org/mediawiki/parts/8/85/T--Edinburgh_OG_BBa_K2739009--image_1.jpg
  
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the effects on PHBV production of having only phaA, only bktBand both two genes
 
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 22:44, 10 October 2018

Hybrid promoter-PhaCAB-Bktb

This is a composite part that was created to allow us to test the functionality of BktB. PHA operon is known to produce bioplastic PHB. The coexpression of the BktB allow the production of copolymer, PHBV.

Usage and Biology

The BktB was isolated from R. eutropha H16 and being recognised as a phaA paralogous, which allows the formation of 3-ketovaleryl-CoA and leads to PHBV production. In order to enhance the 3HV fraction in PHBV, paralog bktB was introduced into E. coli BL21 (DE3) with co-expression of phaCAB operon from Ralstonia eutropha.

T--Edinburgh_OG_BBa_K2739009--image_1.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 979
    Illegal BglII site found at 1804
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 285
    Illegal NgoMIV site found at 356
    Illegal NgoMIV site found at 956
    Illegal NgoMIV site found at 1268
    Illegal NgoMIV site found at 1547
    Illegal NgoMIV site found at 2199
    Illegal NgoMIV site found at 2221
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4065
    Illegal BsaI site found at 5108