Difference between revisions of "Part:BBa K2739009"

Revision as of 22:27, 10 October 2018

Hybrid promoter-PhaCAB-Bktb

This composite part is designed to investigate the effect of PHBV production using PHA synthetic pathway (PHA operon) with BtkB co-expressed. The BktB was isolated from R. eutropha H16 and being recognised as a phaA paralogous, which allows the formation of 3-ketovaleryl-CoA and leads to PHBV production.

Usage and Biology

In order to enhance the 3HV fraction in PHBV, paralog bktB was introduced into E. coli BL21 (DE3) with co-expression of phaCAB operon from Ralstonia eutropha.

the effects on PHBV production of having only phaA, only bktBand both two genes

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 979
Illegal BglII site found at 1804
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 285
Illegal NgoMIV site found at 356
Illegal NgoMIV site found at 956
Illegal NgoMIV site found at 1268
Illegal NgoMIV site found at 1547
Illegal NgoMIV site found at 2199
Illegal NgoMIV site found at 2221
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 4065
Illegal BsaI site found at 5108

@@ Line 12: / Line 12: @@
 In order to enhance the 3HV fraction in PHBV, paralog bktB was introduced into E. coli BL21 (DE3) with co-expression of phaCAB operon from Ralstonia eutropha.
-<img src="https://static.igem.org/mediawiki/2018/2/2e/ T--Edinburgh_OG_BBa_K2739009--image_1.jpg" align="left"  width="600px"/>
+<img src="https://static.igem.org/mediawiki/parts/8/85/T--Edinburgh_OG_BBa_K2739009--image_1.jpg" align="left"  width="600px"/>
   the effects on PHBV production of having only phaA, only bktBand both two genes