Difference between revisions of "Part:BBa K2556032"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This part is mainly composed of three elements: | + | <p>This part is mainly composed of three elements: |
(1)Arabinose induced promoter PBAD to express the gene of downstream.This part contains the promoter as well as the coding sequence for the repressor AraC which is transcribed in the opposite direction. (“upstream”) By binding to L(+)-arabinose, AraC changes its conformation. This causes the protein to diffuses from the DNA thereby inducing transcription. | (1)Arabinose induced promoter PBAD to express the gene of downstream.This part contains the promoter as well as the coding sequence for the repressor AraC which is transcribed in the opposite direction. (“upstream”) By binding to L(+)-arabinose, AraC changes its conformation. This causes the protein to diffuses from the DNA thereby inducing transcription. | ||
(2)This part contains the lac operator as well as the coding sequence for the repressor LacI.The LacI repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D- thiogalactopyranoside (IPTG). | (2)This part contains the lac operator as well as the coding sequence for the repressor LacI.The LacI repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D- thiogalactopyranoside (IPTG). | ||
| − | (3)GFP, as the report gene, can verify the degree of repression by LacI repressor. The lower fluorescence，the higher degree of repression. | + | (3)GFP, as the report gene, can verify the degree of repression by LacI repressor. The lower fluorescence，the higher degree of repression.</p> |
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Revision as of 11:36, 10 October 2018
The lac repressor controled by arabinose binds to the lac operator to inhibit transcription
The lac repressor controlled by arabinose binds to the lac operator to inhibit transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2640
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal BamHI site found at 3159
Illegal XhoI site found at 3060 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961