Difference between revisions of "Part:BBa K2617000"
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===Enzyme activity ===
 
===Enzyme activity ===
  
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[[File:T--UESTC-China-xylanase activity.png|500px|thumb|center|'''Fig. 1''' Xylanase Activity on Xylan .Data were measured in  buffer at pH 6.0 and 40℃.]]
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[[File:T--UESTC-China-standard curve of xylose .png|800px|thumb|center|'''Fig. 1''' Standard Curve of Xylose. Data were measured in  buffer at pH 6.0 and 40℃.]]
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[[File:T--UESTC-China-xylanase activity.png|500px|thumb|center|'''Fig. 2''' Xylanase Activity on Xylan. Data were measured in  buffer at pH 6.0 and 40℃.]]
  
  

Revision as of 10:32, 10 October 2018


Xyn10D-Fae1A：Bifunctional xylanase/ferulic acid esterase

Xyn1D-fae1A cuts off the crosslinking between polysaccharides and lignin in cell wall and degrade xylose hemicellulose. This part is responsible for pretreatment of straw.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 534
    Illegal AgeI site found at 381
    Illegal AgeI site found at 628
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1236

Characterization

Molecular weight

This halohydrin dehalogenase gene codes for a protein of 726 amino acids with a molecular mass of 82.3 kDa.

Enzyme activity

Fig. 1 Standard Curve of Xylose. Data were measured in buffer at pH 6.0 and 40℃.
Fig. 2 Xylanase Activity on Xylan. Data were measured in buffer at pH 6.0 and 40℃.