Difference between revisions of "Part:BBa K2703008:Design"

 
 
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===Design Notes===
 
===Design Notes===
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From the aphVIII gene sequence of the BioBrick BBa_K1884013 (iGEM16_SZU-China) XXX’s restriction site was removed ( G551C) by PCR point mutation. We cloned aphVIII by PCR amplification in B4-B5 position of the MoClo standard2 to use it in our retrotransposon design
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===Source===
 
===Source===
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===References===
 
===References===
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<ol>
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<li> Sizova, I., Fuhrmann, M. & Hegemann, P. A Streptomyces rimosus aphVIII gene coding for a new type phosphotransferase provides stable antibiotic resistance to Chlamydomonas reinhardtii. Gene 277, 221–229 (2001).</li>
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DOI: 10.1021/acssynbio.8b00251 <br>
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<li> Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A modular cloning system for standardized assembly of multigene constructs. PLoS One 6, (2011).</li>
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<li>Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. (2018). doi:10.1021/acssynbio.8b00251 </li>
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</ol>

Latest revision as of 10:22, 10 October 2018


paromycin resistance gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

From the aphVIII gene sequence of the BioBrick BBa_K1884013 (iGEM16_SZU-China) XXX’s restriction site was removed ( G551C) by PCR point mutation. We cloned aphVIII by PCR amplification in B4-B5 position of the MoClo standard2 to use it in our retrotransposon design

Source

The part comes from the Chlamydomonas MoClo tookit (Crozet et al., 2018). We received the part from the lab of the authors

References

  1. Sizova, I., Fuhrmann, M. & Hegemann, P. A Streptomyces rimosus aphVIII gene coding for a new type phosphotransferase provides stable antibiotic resistance to Chlamydomonas reinhardtii. Gene 277, 221–229 (2001).

    2. DOI: 10.1021/acssynbio.8b00251

  2. Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A modular cloning system for standardized assembly of multigene constructs. PLoS One 6, (2011).
  3. Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. (2018). doi:10.1021/acssynbio.8b00251