Difference between revisions of "Part:BBa K2703008:Design"
Saniya kari (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
| − | + | From the aphVIII gene sequence of the BioBrick BBa_K1884013 (iGEM16_SZU-China) XXX’s restriction site was removed ( G551C) by PCR point mutation. We cloned aphVIII by PCR amplification in B4-B5 position of the MoClo standard2 to use it in our retrotransposon design | |
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===Source=== | ===Source=== | ||
Revision as of 10:22, 10 October 2018
paromycin resistance gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
From the aphVIII gene sequence of the BioBrick BBa_K1884013 (iGEM16_SZU-China) XXX’s restriction site was removed ( G551C) by PCR point mutation. We cloned aphVIII by PCR amplification in B4-B5 position of the MoClo standard2 to use it in our retrotransposon design
Source
The part comes from the Chlamydomonas MoClo tookit (Crozet et al., 2018). We received the part from the lab of the authors