Difference between revisions of "Part:BBa K2541303:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | The pseudoknot structure is important to the function of cold-inducible RNA thermosensors. We designed the thermosensor by increasing or decreasing the base pairings and GC content in the pseudoknot. | |
+ | ===Source=== | ||
+ | The natural cspA gene sequence is from Escherichia coli. The sequence we used is the 5'UTR of cspA. And we incrase or decrease the base pairings and GC centent of this sequence. We synthesized this sequence from a synthesis company. | ||
+ | ===References=== | ||
+ | [1]Giuliodori A M, Di P F, Marzi S, et al. The cspA mRNA is a thermosensor that modulates translation of the cold-shock protein CspA.[J]. Molecular Cell, 2010, 37(1):21-33. | ||
− | + | [2]Breaker R R. RNA Switches Out in the Cold[J]. Molecular Cell, 2010, 37(1):1-2. | |
− | + | [3]Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches.[J]. Nature Reviews Microbiology, 2012, 10(4):255-65. | |
− | + | ||
− | + |
Revision as of 07:04, 10 October 2018
Cold-inducible RNA-based thermosensor-3
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The pseudoknot structure is important to the function of cold-inducible RNA thermosensors. We designed the thermosensor by increasing or decreasing the base pairings and GC content in the pseudoknot.
Source
The natural cspA gene sequence is from Escherichia coli. The sequence we used is the 5'UTR of cspA. And we incrase or decrease the base pairings and GC centent of this sequence. We synthesized this sequence from a synthesis company.
References
[1]Giuliodori A M, Di P F, Marzi S, et al. The cspA mRNA is a thermosensor that modulates translation of the cold-shock protein CspA.[J]. Molecular Cell, 2010, 37(1):21-33.
[2]Breaker R R. RNA Switches Out in the Cold[J]. Molecular Cell, 2010, 37(1):1-2.
[3]Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches.[J]. Nature Reviews Microbiology, 2012, 10(4):255-65.