Difference between revisions of "Part:BBa K2570010:Design"
| (One intermediate revision by one other user not shown) | |||
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | The phenyl pyruvate decarboxylase from S. cerevisiae (Aro10) | + | The phenyl pyruvate decarboxylase from ''S. cerevisiae'' (Aro10) |
https://www.ncbi.nlm.nih.gov/protein/NP_010668.3?report=genbank&log$=protalign&blast_rank=1&RID=V0V04K3B015 | https://www.ncbi.nlm.nih.gov/protein/NP_010668.3?report=genbank&log$=protalign&blast_rank=1&RID=V0V04K3B015 | ||
| Line 21: | Line 21: | ||
https://www.ncbi.nlm.nih.gov/protein/BAG13450.2?report=genbank&log$=protalign&blast_rank=1&RID=V0NV9P35014 | https://www.ncbi.nlm.nih.gov/protein/BAG13450.2?report=genbank&log$=protalign&blast_rank=1&RID=V0NV9P35014 | ||
| − | The aromatic transaminase from E. coli (TyrB) | + | The aromatic transaminase from ''E. coli'' (TyrB) |
https://www.ncbi.nlm.nih.gov/protein/WP_032305522.1?report=genbank&log$=protalign&blast_rank=3&RID=V13P17MW014 | https://www.ncbi.nlm.nih.gov/protein/WP_032305522.1?report=genbank&log$=protalign&blast_rank=3&RID=V13P17MW014 | ||
| Line 27: | Line 27: | ||
===References=== | ===References=== | ||
| + | Wang, P., Yang, X., Lin, B., Huang, J., & Tao, Y. (2017). Cofactor self-sufficient whole-cell biocatalysts for the production of 2-phenylethanol. Metabolic Engineering, 44(August), 143–149. https://doi.org/10.1016/j.ymben.2017.09.013 | ||
Latest revision as of 05:38, 10 October 2018
Production of 2-PE: Aro10+PAR+TyrB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2242
Illegal NotI site found at 2605 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 4044
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Glutamate dehydrogenase plays a key role in 2-PE biosynthesis.
Source
The phenyl pyruvate decarboxylase from S. cerevisiae (Aro10)
the phenyla-cetaldehyde reductase from the rose (PAR)
The aromatic transaminase from E. coli (TyrB)
References
Wang, P., Yang, X., Lin, B., Huang, J., & Tao, Y. (2017). Cofactor self-sufficient whole-cell biocatalysts for the production of 2-phenylethanol. Metabolic Engineering, 44(August), 143–149. https://doi.org/10.1016/j.ymben.2017.09.013