Difference between revisions of "Help:Spring 2008 DNA distribution"
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Each part in the Registry was assigned a well in a specific 96 well staging plate according to its antibiotic resistance and its base pair length. This grouping made it easier to examine the parts against their known antibiotic resistance and to then sequence their DNA. | Each part in the Registry was assigned a well in a specific 96 well staging plate according to its antibiotic resistance and its base pair length. This grouping made it easier to examine the parts against their known antibiotic resistance and to then sequence their DNA. | ||
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| + | ===Streaking for Single Colonies=== | ||
==Instruction on usage== | ==Instruction on usage== | ||
Revision as of 14:21, 1 May 2008
Contents
iGEM 2008 DNA Parts Kit
Background on 08 distribution
Quality Control Process
This year the Registry has undertaken extensive quality control measures in order to better ensure the accuracy of DNA parts sent to the iGEM 2008 teams. This QC process began in February and has examined each part currently in the Registry to determine if the plasmid and the part within are correct according to the documentation provided with each submission. The results for each part were then posted on the Registry's DNA repository page [ ], allowing iGEM teams to access this information before using the submissions to create their own parts.
Grouping of parts for QC project
Each part in the Registry was assigned a well in a specific 96 well staging plate according to its antibiotic resistance and its base pair length. This grouping made it easier to examine the parts against their known antibiotic resistance and to then sequence their DNA.
Streaking for Single Colonies
Instruction on usage
- punching out spots
- transforming
- picking single colony
- making glycerol
- finding part location