Difference between revisions of "Part:BBa K2570016:Design"

 
 
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===Design Notes===
 
===Design Notes===
−
We did site-directed mutation for hns in order to follow the part-submitted rules.
+
H-NS is a global-regulated protein which could bind to genome and specifically inhibit ''proU''. We did site-directed mutation for ''hns'' in order to meet the requirements of RFC10. Detailed information in [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2570011 BBa_K2570011]
  
  
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===Source===
 
===Source===
  
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It comes from E.coli.
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''E.coli K12''.
  
 
===References===
 
===References===
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[1]Interactions of the Nucleoid-associated DNA-binding Protein H –NS with the Regulatory Region of the Osmotically Controlled proU Operon of Escherichia coli* The JOUTNAL OF BIOLOGICAL CHEMISTRY, April 1994

Latest revision as of 04:19, 10 October 2018


hns


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 154


Design Notes

H-NS is a global-regulated protein which could bind to genome and specifically inhibit proU. We did site-directed mutation for hns in order to meet the requirements of RFC10. Detailed information in BBa_K2570011


Source

E.coli K12.

References

[1]Interactions of the Nucleoid-associated DNA-binding Protein H –NS with the Regulatory Region of the Osmotically Controlled proU Operon of Escherichia coli* The JOUTNAL OF BIOLOGICAL CHEMISTRY, April 1994