Difference between revisions of "Part:BBa K2574000"

Latest revision as of 03:32, 10 October 2018

VLP forming Gag Construct from Maize CR element.

The 2018 Hawaii iGEM team designed a DNA sequence that codes for the proteins necessary for the formation of Virus-Like Particles. Gag; the structure forming unit of retroviral and retroviral-like elements, is further divided into the matrix, capsid, and nucleocapsid domains. The make-up and function of Gag domains varies widely between retroviral and retroviral-like elements. Our design derives from a Maize centromeric retrotransposon (CR) element. Our sequence also contains a Histidine tag at the 5' end which is used in DNA purification using a nickel based column.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 02:38, 10 October 2018 (view source) Registry (Talk \| contribs)		Latest revision as of 03:32, 10 October 2018 (view source) Jtello2 (Talk \| contribs)
Line 3:		Line 3:
	<partinfo>BBa_K2574000 short</partinfo>		<partinfo>BBa_K2574000 short</partinfo>

−	The 2018 Hawaii iGEM team designed a DNA sequence that codes for the proteins necessary for the formation of Virus-Like Particles. Gag; the structure forming unit of retroviral and retroviral-like elements, is further divided into the matrix, capsid, and nucleocapsid domains. The make-up and function of Gag domains varies widely between retroviral and retroviral-like elements. Our design derives from a Maize centromeric retrotransposon (CR) element.	+	The 2018 Hawaii iGEM team designed a DNA sequence that codes for the proteins necessary for the formation of Virus-Like Particles. Gag; the structure forming unit of retroviral and retroviral-like elements, is further divided into the matrix, capsid, and nucleocapsid domains. The make-up and function of Gag domains varies widely between retroviral and retroviral-like elements. Our design derives from a Maize centromeric retrotransposon (CR) element. Our sequence also contains a Histidine tag at the 5' end which is used in DNA purification using a nickel based column.