Difference between revisions of "Part:BBa K2609000"

 
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<partinfo>BBa_K2609000 short</partinfo>
 
<partinfo>BBa_K2609000 short</partinfo>
  
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Member of CC chemokine family that uses the CCR2 receptor for chemo-attracting monocytes to the site of release. Is non-glycosylated and does not require any post translational modfications and thus can be recombinantly expressed in E. coli.
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Member of CC chemokine family that uses the CCR2 receptor for chemo-attracting monocytes to the site of release (monocyte chemoattractant protein - 1). This coding sequence lacks the heavily glycosylated C-terminus present in the wild-type protein and has been shown to have a increased chemotactic potency<sup>[1][2]</sup>. The protein can be expressed recombinantly in a prokaryotic system because of its lack of glycosylation and post-translational modifications.
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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<html>
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<h2>Biology</h2>
  
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<h2>Usage</h2>
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</html>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2609000 parameters</partinfo>
 
<partinfo>BBa_K2609000 parameters</partinfo>
 
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===References===
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[1] Yao, Yao, and Stella E. Tsirka. "Mouse monocyte chemoattractant protein 1 (MCP1) functions as a monomer." The international journal of biochemistry & cell biology 55 (2014): 51-59.
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[2] Deshmane, Satish L., et al. "Monocyte chemoattractant protein-1 (MCP-1): an overview." Journal of interferon & cytokine research 29.6 (2009): 313-326.

Revision as of 19:49, 9 October 2018


Murine JE/MCP-1

Member of CC chemokine family that uses the CCR2 receptor for chemo-attracting monocytes to the site of release (monocyte chemoattractant protein - 1). This coding sequence lacks the heavily glycosylated C-terminus present in the wild-type protein and has been shown to have a increased chemotactic potency[1][2]. The protein can be expressed recombinantly in a prokaryotic system because of its lack of glycosylation and post-translational modifications.


Usage and Biology

Biology

Usage

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Yao, Yao, and Stella E. Tsirka. "Mouse monocyte chemoattractant protein 1 (MCP1) functions as a monomer." The international journal of biochemistry & cell biology 55 (2014): 51-59.

[2] Deshmane, Satish L., et al. "Monocyte chemoattractant protein-1 (MCP-1): an overview." Journal of interferon & cytokine research 29.6 (2009): 313-326.