Difference between revisions of "Part:BBa K2686002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The HexaHistidine sequence was codon optimized for bacterial expression in E. coli | + | The HexaHistidine sequence was codon optimized for bacterial expression in E. coli. |
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===Source=== | ===Source=== | ||
Revision as of 18:17, 9 October 2018
Encapsulin protein with HexaHistidine insert
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 77
Illegal BglII site found at 492 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 426
Illegal SapI.rc site found at 457
Design Notes
The HexaHistidine sequence was codon optimized for bacterial expression in E. coli.
Source
Derived from BBa_K2686001.
References
Moon, H., Lee, J., Min, J. and Kang, S. (2014). Developing Genetically Engineered Encapsulin Protein Cage Nanoparticles as a Targeted Delivery Nanoplatform. Biomacromolecules, 15(10), pp.3794-3801.