Difference between revisions of "Part:BBa I715054"
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This part is designed to test the functionality of the <i>trp</i> operon regulatory sequence which uses transcriptional attenuation (via the "anti-termination" mechanism) to regulate gene expression.
  
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===Usage and Biology===
 
===Usage and Biology===
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In the presence of tryptophan, the 'trp' regulatory sequence will attenuate transcription, and GFP will not be transcribed.  This construct serves as a means of testing the functionality of the 'trp' regulatory sequence by comparing GFP expression with different environmental concentrations of tryptophan amino acid.
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In the presence of tryptophan, the <i>trp</i> regulatory sequence will attenuate transcription, and GFP will not be transcribed.  This construct serves as a means of testing the functionality of the 'trp' regulatory sequence by comparing GFP expression with different environmental concentrations of tryptophan amino acid.
  
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_I715054 parameters</partinfo>
 
<partinfo>BBa_I715054 parameters</partinfo>
 
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Revision as of 17:31, 23 March 2008

Test Trp anti-terminator/terminator

This part is designed to test the functionality of the trp operon regulatory sequence which uses transcriptional attenuation (via the "anti-termination" mechanism) to regulate gene expression.

Usage and Biology

In the presence of tryptophan, the trp regulatory sequence will attenuate transcription, and GFP will not be transcribed. This construct serves as a means of testing the functionality of the 'trp' regulatory sequence by comparing GFP expression with different environmental concentrations of tryptophan amino acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1012


Functional Parameters