Difference between revisions of "Part:BBa K2804001:Design"
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===Source=== | ===Source=== | ||
| − | + | Fusion assembly. | |
===References=== | ===References=== | ||
New England Biolabs. Cleavage Close to the End of DNA Fragments (oligonucleotides). https://www.neb.com/-/media/nebus/files/chart-image/cleavage_olignucleotides_old.pdf?la=en | New England Biolabs. Cleavage Close to the End of DNA Fragments (oligonucleotides). https://www.neb.com/-/media/nebus/files/chart-image/cleavage_olignucleotides_old.pdf?la=en | ||
Revision as of 16:16, 9 October 2018
CBD cipA fused to BMP2 under the control of LacI promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 230
Illegal AgeI site found at 1106 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
It was necessary to add basepairs at the N-terminus and C-terminus of the two gBlocks (CBD cipA under the control of LacI promoter and BMP2) in order to allow the cleavage by EcoRI and PstI. Then, a 3A assembly was done using the psb1c3 as cloning vectorof the fusion CBD cipA-BMP2.
Source
Fusion assembly.
References
New England Biolabs. Cleavage Close to the End of DNA Fragments (oligonucleotides). https://www.neb.com/-/media/nebus/files/chart-image/cleavage_olignucleotides_old.pdf?la=en