Difference between revisions of "Part:BBa K2703002:Experience"
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was as a reporter gene. After transformation in C.reinhardtii D66 the functionality of the promoter was tested by counting the number of chlamydomonas colony resistant to paromomycine (15 ug/ml). Different constructions were made to characterize and compare the functionality of P pSAD . We tested different combination of promoters and 3’UTR. | was as a reporter gene. After transformation in C.reinhardtii D66 the functionality of the promoter was tested by counting the number of chlamydomonas colony resistant to paromomycine (15 ug/ml). Different constructions were made to characterize and compare the functionality of P pSAD . We tested different combination of promoters and 3’UTR. | ||
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<td>Literrature researches</td> | <td>Literrature researches</td> | ||
<td>Whole team participated.</td> | <td>Whole team participated.</td> | ||
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<td>Modelisation</td> | <td>Modelisation</td> | ||
<td>Victor Sayous, Ursula Saade, Charlotte Bellamy </td> | <td>Victor Sayous, Ursula Saade, Charlotte Bellamy </td> | ||
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Revision as of 14:45, 9 October 2018
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This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2703002
Our part is compatible with the PhytoBrick MoClo standard. This PhytoBrick was intented to be use as constitutive promoter for the transcription unit of paromomycine in the CARGO of our synthetic retrotransposons for directed evolution on the Chlamydomoas. It is part of our reporter system to characterize the activity and transposition rate.
We couldn’t directly use our Phytobrick for the characterisation because we didn’t have time to construct all the other that are needed for the final assembly. Instead we use a slightly different Phytobrick with the same P PSAD. sequence but with one different fusion site. This part was assembled in a functional Transcription Unit with the resistance gene paromomycine that
was as a reporter gene. After transformation in C.reinhardtii D66 the functionality of the promoter was tested by counting the number of chlamydomonas colony resistant to paromomycine (15 ug/ml). Different constructions were made to characterize and compare the functionality of P pSAD . We tested different combination of promoters and 3’UTR.
| Literrature researches | Whole team participated. | ||||||||
| Protocols | Aurélie Bouin | ||||||||
| Shipping lab materials | Lucas Leonardi, Saniya Kari | ||||||||
| Interlab | Ursula Saade, Asmaa Foda and Dounia Chater | ||||||||
| "Safety form | Aurélie Bouin and Saniya Kari | ||||||||
| Modelisation | Victor Sayous, Ursula Saade, Charlotte Bellamy |
User Reviews
UNIQa4ca276e393f3c21-partinfo-00000000-QINU UNIQa4ca276e393f3c21-partinfo-00000001-QINU