Difference between revisions of "Part:BBa K2610015"
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The YmfM gene codes for hypothetical protein YmfM. | The YmfM gene codes for hypothetical protein YmfM. | ||
| + | ===Usage and Biology=== | ||
| + | <p>iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. | ||
| + | We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours. </p> <br> | ||
| + | |||
| + | [[File:T--Leiden--heatmap.png|750px]] <br> | ||
| + | <span style="font-size:1em"><b>Figure 1.</b> Heatmap with stress-activated promoters </span> | ||
| + | |||
| + | As can be observed in Figure 1 the YmfM promoter is only moderately activated by tetracycline. | ||
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Latest revision as of 14:42, 9 October 2018
pYmfM
The YmfM gene codes for hypothetical protein YmfM.
Usage and Biology
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours.
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the YmfM promoter is only moderately activated by tetracycline.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]