Difference between revisions of "Part:BBa K2848001:Design"
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The primer for adding the His-Tag was provided with an additional SpeI restriction site. | The primer for adding the His-Tag was provided with an additional SpeI restriction site. | ||
| − | + | PCR using BBa_K2380043 as template and the primers VF2 (5'-tgccacctgacgtctaagaa-3') and COD_rev+His6+SpeI (5'-[GCCGCTACTAGTATTAGTGGTGATGGTGATGATG]cagagcagtaaacagcgtattctggg-3'). | |
===Source=== | ===Source=== | ||
| − | iGEM Team Darmstadt 2017 ( | + | iGEM Team Darmstadt 2017 (ChiTUcare) |
===References=== | ===References=== | ||
Latest revision as of 14:05, 9 October 2018
NodC+COD-HisTag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1613 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
The primer for adding the His-Tag was provided with an additional SpeI restriction site.
PCR using BBa_K2380043 as template and the primers VF2 (5'-tgccacctgacgtctaagaa-3') and COD_rev+His6+SpeI (5'-[GCCGCTACTAGTATTAGTGGTGATGGTGATGATG]cagagcagtaaacagcgtattctggg-3').
Source
iGEM Team Darmstadt 2017 (ChiTUcare)