Difference between revisions of "Part:BBa K2610007"
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MicF small RNA, coded by the MicF gene, is produced as stress response in Escherichia coli. It negatively regulates translation of the OmpF porin. MicF binds to OmpF mRNA and represses production of OmpF in response to environmental and internal stresses. The MicF expression is stimulated by many factors such as oxidative stress, osmolarity increase and temperature increase. | MicF small RNA, coded by the MicF gene, is produced as stress response in Escherichia coli. It negatively regulates translation of the OmpF porin. MicF binds to OmpF mRNA and represses production of OmpF in response to environmental and internal stresses. The MicF expression is stimulated by many factors such as oxidative stress, osmolarity increase and temperature increase. | ||
| + | ===Usage and Biology=== | ||
| + | <p>iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) </p> <br> | ||
| + | |||
| + | [[File:T--Leiden--heatmap.png|750px]] <br> | ||
| + | <span style="font-size:1em"><b>Figure 1.</b> Heatmap with stress-activated promoters </span> | ||
| + | |||
| + | As can be observed in Figure 1 the MicFpromoter is only moderately activated by tetracycline | ||
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Revision as of 13:00, 9 October 2018
pMicF
MicF small RNA, coded by the MicF gene, is produced as stress response in Escherichia coli. It negatively regulates translation of the OmpF porin. MicF binds to OmpF mRNA and represses production of OmpF in response to environmental and internal stresses. The MicF expression is stimulated by many factors such as oxidative stress, osmolarity increase and temperature increase.
Usage and Biology
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1)
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the MicFpromoter is only moderately activated by tetracycline
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]