Difference between revisions of "Part:BBa K2690002"

Latest revision as of 12:44, 9 October 2018

PvsB - Vibrio parahaemolyticus vibrioferrin synthesis gene B

This coding sequence is part of the biosynthetic gene cluster for the synthesis and transport of the siderophore vibrioferrin. It's specific function in this process to to code for an enzyme for amide bond formation between 2-ketoglutarate and L-alanine, or L-alanine and ethanolamine (Fujita et al., 2011).

Primers for Biobrick construction and PCR amplification.

Forward Primer

5’ – GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAT CAC AAC GCT TTA TAC CTA ACC– 3’

Reverse Primer

5’ – GT TTC TTC CTG CAG CGG CCG CTA CTA GTA TTA TTA ACG TAC GCT TTT TTT GAT GGC – 3’

Notes: forward G-C content = 25/55 = 45%; reverse G-C content = 25/56 = 46%

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 555
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 684
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 This coding sequence is part of the biosynthetic gene cluster for the synthesis and transport of the siderophore vibrioferrin. It's specific function in this process to to code for an enzyme for amide bond formation between 2-ketoglutarate and L-alanine, or L-alanine and ethanolamine (Fujita et al., 2011).
-<!-- Add more about the biology of this part here
+Primers for Biobrick construction and PCR amplification.
-===Usage and Biology===
-Priers for Biobrick construction and PCR amplification.
 Forward Primer
@@ Line 19: / Line 16: @@
 Notes: forward G-C content = 25/55 = 45%; reverse G-C content = 25/56 = 46%
+<!-- Add more about the biology of this part here
+===Usage and Biology===
 <!-- -->