Difference between revisions of "Part:BBa K2610003"
| Line 5: | Line 5: | ||
Small heat shock protein IbpB is involved in heat shock response and oxidative stress. As ibpA, it is involved in substrate refolding in cooperation with ATP-dependent chaperones, such as DnaK and ClpB. | Small heat shock protein IbpB is involved in heat shock response and oxidative stress. As ibpA, it is involved in substrate refolding in cooperation with ATP-dependent chaperones, such as DnaK and ClpB. | ||
| + | ==Usage and Biology=== | ||
| + | <p>iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) </p> <br> | ||
| − | + | [[File:T--Leiden--heatmap.png|750px]] <br> | |
| − | = | + | <span style="font-size:1em"><b>Figure 1.</b> Heatmap with stress-activated promoters </span> |
| + | As can be observed in Figure 1 the IbpB promoter is only moderately activated by tetracycline | ||
| + | |||
| + | <!-- Add more about the biology of this part here | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 12:32, 9 October 2018
pIbpB
Small heat shock protein IbpB is involved in heat shock response and oxidative stress. As ibpA, it is involved in substrate refolding in cooperation with ATP-dependent chaperones, such as DnaK and ClpB.
Usage and Biology=
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1)
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the IbpB promoter is only moderately activated by tetracycline
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]