Difference between revisions of "Part:BBa K2610002"

(Usage and Biology)
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__NOTOC__
 
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<partinfo>BBa_K2610002 short</partinfo>
 
<partinfo>BBa_K2610002 short</partinfo>
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<p>Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli. </p><br>
  
 
===Usage and Biology===
 
===Usage and Biology===
<p>Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli.<br> <br> iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated against a repertoire of known antibiotics (Figure 1) </p> <br>
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<p>iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated against a repertoire of known antibiotics (Figure 1) </p> <br>
  
 
[[File:T--Leiden--heatmap.png|750px]] <br>
 
[[File:T--Leiden--heatmap.png|750px]] <br>

Revision as of 12:22, 9 October 2018


pIbpA

Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli.


Usage and Biology

iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated against a repertoire of known antibiotics (Figure 1)


T--Leiden--heatmap.png
Figure 1. Heatmap with stress-activated promoters

As can be observed in Figure 1 the ibpA promoter is only moderately activated by streptomycin and tetracycline.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]